AMR Basics Food Safety Network Pharmacology Survey Health Tools Medicines Research
πŸ”¬ Research 2026 Β· Open to Everyone

ML-Assisted Probiotic Research & AMR Awareness Platform

Understanding antibiotic resistance is everyone's responsibility β€” students, families, communities. This platform explains AMR in simple words, lets you test your knowledge, and connects you to cutting-edge pharmaceutical research.

33+
Publications
50+
Impact Factor
15+
Years
3
Collaborators
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ML-Assisted Network Pharmacology

AI maps probiotic compounds to resistance targets β€” predicting which strains beat AMR pathogens

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In Vitro Assays

Lab-based antimicrobial and antibiofilm testing

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AMR Surveillance

Pakistan & global resistance awareness

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Get Your Personal AMR Risk Score

Fill the survey β†’ instant personalised report shared back to you

⚠️ Simple Explanation β€” Everyone Should Know

What is Antibiotic Resistance?

You don't need to be a doctor to understand this. These are simple, clear explanations for everyone β€” students, parents, teachers, and the general public.

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AMR β€” In Simple Words

Imagine giving a painkiller to someone who feels no pain from it anymore. AMR means bacteria have become so used to antibiotics that the medicine no longer kills them. The bacteria survive and multiply β€” making you very sick.

Everyone is at risk
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MDR β€” When Many Medicines Stop Working

MDR means "Multi-Drug Resistant" β€” the bacteria resist 3 or more types of antibiotics at the same time. In Pakistan, many common infections like TB, typhoid, and UTIs are now MDR. Doctors run out of treatment options.

Pakistan hotspot
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XDR β€” Extremely Resistant

XDR means "Extensively Drug Resistant" β€” almost NO antibiotic works. Pakistan had the world's first XDR typhoid outbreak in 2016. Only 1 or 2 very expensive medicines were left β€” and many families could not afford them.

Pakistan origin 2016
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Are YOU Causing Resistance?

Yes, if you:
β€’ Buy antibiotics without a doctor's prescription
β€’ Stop taking antibiotics when you feel better
β€’ Take leftover antibiotics for a new illness
β€’ Share your antibiotics with family members

All of these common habits create resistant bacteria.

Very common in Pakistan
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How Probiotics Help

Probiotic bacteria produce natural substances that can kill resistant bacteria. Our research tests these probiotic strains in the lab to find new medicines β€” without chemicals that cause resistance.

Our research focus
βœ…

3 Things You Can Do Today

1. Only take antibiotics prescribed by a doctor.

2. Always finish the full antibiotic course β€” even if you feel better after 2 days.

3. Eat probiotic-rich foods like dahi and lassi β€” they protect your gut and help your immune system fight infections naturally.

Act now
🍽️ Food Safety & Foodborne Pathogens

Is Your Food Making Resistant Bacteria Stronger?

Foodborne bacteria are one of the biggest β€” and least talked about β€” routes for antimicrobial resistance to spread. Here's what the science says and how to protect your family.

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What Are Foodborne Pathogens?

Bacteria that contaminate food and cause illness β€” commonly found in undercooked meat, unpasteurised dairy, and improperly handled street food. Many of the strains circulating in Pakistan are now multidrug-resistant (MDR).

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How We Confirm Them

Our lab uses species-specific real-time PCR to confirm both pathogen identity and resistance/virulence genes directly from food samples β€” faster and more specific than traditional culture methods.

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The Scale of the Problem

WHO estimates roughly 600 million foodborne illness episodes and 420,000 deaths every year worldwide, with South Asia disproportionately affected by outbreaks linked to retail meat and street food.

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Higher-Risk Foods

  • Undercooked meat and poultry
  • Unpasteurised milk and dairy
  • Cut fruit left at room temperature
  • Reheated rice (linked to Bacillus cereus)
  • Street food with poor hand hygiene
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WHO's 5 Keys to Safer Food

  • Keep clean
  • Separate raw and cooked
  • Cook thoroughly
  • Keep food at safe temperatures
  • Use safe water and raw materials
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Our Plant-Based Research

Extracts from Punjab's medicinal plants β€” screened against PCR-confirmed foodborne MDR pathogens in our lab β€” have shown strong inhibitory and antibiofilm activity, including against resistant Salmonella and S. aureus strains, offering early leads for future food-safe antimicrobials.

Salmonella enterica Staphylococcus aureus (MRSA) Escherichia coli Shigella sonnei Bacillus cereus Candida albicans
πŸ”¬ The Science Behind Our Research

What is Network Pharmacology?

Our ML Research Pipeline

🍢Isolate probiotic strains
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πŸ”Identify active compounds in probiotics
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πŸ•ΈοΈMap compounds β†’ disease targets (Network)
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πŸ€–ML model predicts which compound kills MDR bacteria
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🧫Validate predictions in real lab experiments
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πŸ’ŠExplore translation into a topical antimicrobial formulation

Explained Simply

Network Pharmacology is like Google Maps for drug discovery. Instead of testing one drug on one target, it maps all possible connections between bioactive compounds and disease-causing bacteria β€” finding the best route to defeat AMR.

We then use Machine Learning (AI) to learn from thousands of data points and predict which strains and compounds will be most effective against resistant bacteria β€” before we even test them in the lab. This saves time, money and resources.

In vitro lab tests then confirm the computational predictions, and active leads are carried forward for further pharmacological characterisation (MIC, MBC, biofilm eradication) and molecular docking.

This combined approach is faster, cheaper and more accurate than traditional trial-and-error drug discovery.

πŸ“‹ Research Participation

AMR Awareness Survey

Open to everyone β€” any age, any background, any field of study or work. Takes 5 minutes. You get your personal AMR risk score and recommendations instantly.

🌍 This survey is open to all students and the general public β€” any university, any department, any age. Your participation helps our published research and you receive personalised health feedback instantly.
Step 1 of 4 β€” Your Details
Tell us a little about yourself
~5 minutes Β· 10 questions Β· Instant results
Question 1 of 10
Section 3 β€” Your Health Habits
Tell us about your antibiotic and food habits
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Survey Complete!

Here is your personal AMR Awareness Report

0/10
βœ… Thank you! Your results have been noted by our research team a detailed report will be sent to your email. Your anonymous data helps advance AMR research in Pakistan.
🩺 Health Tools
🩺 Free Health Tools

Personal Health Calculators

Free tools for everyone. For educational purposes β€” always consult a qualified healthcare provider for medical decisions.

βš–οΈ BMI Calculator

Enter weight and height above

πŸ”₯ Daily Calorie Needs

Fill all fields above

πŸ’Š Antibiotic Risk Checker

Check if your antibiotic habits put you at AMR risk

Answer all questions above

🍢 Probiotic Food Advisor

Find the best probiotic-rich foods from Pakistani diet

Select your goal and preference
πŸ’Š Medicine Education

Common Antibiotics β€” What You Should Know

Educational information about commonly used antibiotics in Pakistan. This is for awareness only

πŸ”¬ Active Research Project 2026

Our Research

ML-Assisted Network Pharmacology and In Vitro Validation of Bioactive Probiotics Against Antimicrobial-Resistant Pathogens: A Digital Community Engagement and Awareness Platform

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Probiotic Isolation

Isolate bioactive strains

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Compound Identification

Identify active bioactive compounds from isolated strains

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Network Pharmacology + ML

AI maps compounds to AMR targets β€” predicts best candidates

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In Vitro Validation

confirm AI predictions in lab

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Translational Formulation

Exploring a topical antimicrobial formulation with industry partner Zordic Pharma β€” early-stage development

🧫 Second Active Research Strand 2026

Food Microbiology: Real-Time PCR-Based Pathogen Research

Antimicrobial and Antibiofilm Activity of Twenty-Five Ethnomedicinal Plants from Punjab, Pakistan Against Real-Time PCR-Confirmed Foodborne MDR Pathogens: MIC, MBC, MBEC Determination and Molecular Docking of Phytoligands

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Food & Plant Collection

Food samples from 17 Punjab cities; 25 medicinal plants from 8 districts

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Real-Time PCR Confirmation

Species identity + resistance/virulence genes (invA, stx1/stx2, mecA/nuc, ERG11)

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Antimicrobial & Antibiofilm Screening

MIC, MBC, BIC and MBEC determination against PCR-confirmed pathogens

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Molecular Docking

133 Lipinski-filtered phytoligands screened against 5 AMR protein targets

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Lead Identification

Tecomella undulata fractions and solamargin/Ξ±-lapachone as priority anti-AMR leads